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. 2021 May 23;12(3):1155–1158.e4. doi: 10.1016/j.jcmgh.2021.05.009

Figure 1.

Figure 1

Targeting a CreER-2A-tdTomato reporter to the endogenous FoxL1 locus. (A) Targeting vector for inserting a CreER-2A-tdTomato cassette at the translational start site of the endogenous FoxL1 locus. Primer locations for validating insertion at the 5’ and 3’ insertion sites are indicated, as are primers used for genotyping of FoxL1CreER-2A-Tomato mice (A, B, and C). (B) Polymerase chain reaction assays spanning the 5’ and 3’ arms of homology validate proper gene targeting. (C) Genotyping assay for the FoxL1CreER-2A-Tomato mouse strain using a 3-primer (A, B, and C) polymerase chain reaction enables resolution of wild-type (WT) and targeted (Mut) FoxL1 alleles. (D) Endogenous tdTomato expression in the subepithelial telocyte plexus in the jejunum of FoxL1CreER-2A-Tomato mice. (E) Sun1GFP expression in a FoxL1CreER-tdTomRosa26Sun1GFP/+ mouse after 5 doses of tamoxifen . (F) Overlap of endogenous tdTomato and Sun1GFP expression. PGK, phosphoglycerate kinase I.