Supplementary Figure 2.

EGFR localization in normal duodenum in Lrig1^Ap/+;Egfr^Em/+and Lrig1^Ap/Ap;Egfr^Em/+mice. Immunofluorescence for Egfr^Em (green, anti-GFP staining) and Lrig1^Ap (red, endogenous fluorescence) in normal duodenum from Lrig1^Ap/+;Egfr^Em/+ (A) and Lrig1^Ap/Ap; Egfr^Em/+ (B) mice. Arrows and arrowheads indicate Egfr^Em/Lrig1^Ap-positive cells, and Egfr^Em-positive and Lrig1^Ap-negative stroma, respectively. Scale bar = 100 μm.