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. 2021 Sep 2;11:17542. doi: 10.1038/s41598-021-97038-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of VLPs derived from all serotypes of DENV. (a) E protein structures of DENV-2, DENV-3 and DENV-4 were constructed with amino acid sequence of aa 1–395, aa 1–392 and aa 1–395, respectively. Positions of FLAG tag insertion at 273/274 in DENV-2 and -4 E protein and at 271/272 in DENV-3 E protein were shown with red circles. (b) Protein expression of FLAG inserted DENV-LPs derived from serotypes 1 (D1), 2 (D2), 3 (D3) and 4 (D4) VLP in cell lysates (left) and culture supernatants (right) were detected by western blotting. Non-transfected HEK293T samples (293 T) were added as negative controls. Amounts of cell lysates from D1 and D2 samples used were one-tenth compared to those from D3 and D4 as well as negative control. (c) Protein expression of DENV-LPs derived from serotypes 1, 2, 3 and 4 without FLAG tag in cell lysates (left) and culture supernatants (right) were detected by western blotting. Non-transfected HEK293T samples (293 T) were added as negative controls. Uncropped images of western blotting pictures in Fig. 2 are included in supplementary Fig. 2.