Table 3.

Performance of objectwise validation.

3D-stack	TP	FN	FP	Sensitivity	Precision	F1
Original	472	19	33	0.96	0.93	0.95
Filtered	368	6	29	0.98	0.93	0.95

Validation of segmentation of pyramidal cells based on 3D-reconstruction. Precision, sensitivity, and F1-scores were calculated based on 491 reconstructed pyramidal cells from 30 stacked 2048 × 3840 manually marked images. Original refers to the case where centroid points from all 30 images are included. Filtered refers to the case where centroid points from the first and last three images of the stack were excluded.