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. 2021 Sep 2;11:17626. doi: 10.1038/s41598-021-96863-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Frequency distribution of P_i values based on the results from positive and negative datasets of bacteria, protozoa, fungi, and viruses. The Y-axis represents sequence count. The X-axis represents the *P_i score values for each sequence. Blue depicts non-antigen and red antigen sequences. *P_i stands for probability value where P_i = S_i/N (where, S_i refers to the row-wise sum values and N refers to the total number of the tools).