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. 2021 Sep 2;11:17587. doi: 10.1038/s41598-021-97060-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of interaction region(s) of CXXC5 with MeCP2. (a) Schematics of CXXC5 variants. 3F denotes 3XFlag tag and eNLS is an NLS signal derived from the SV40 T antigen. DBM denotes DNA binding mutant. (b) The synthesis of proteins in transiently transfected HEK293 cells was verified by WB using the Flag antibody. Molecular masses (MM) in kDa are indicated. (c) Transiently transfected HEK293 cells were subjected to ICC using the Flag antibody followed by Alexa Fluor 488 conjugated secondary antibody for visualization with a fluorescence microscope. DAPI was used for the nuclei staining. (d) HEK293 cells were transiently co-transfected with the expression vector bearing cDNA for a 3F-CXXC5 variant and HA-MeCP2. Nuclear extracts (500 ug) were subjected to Co-IP with the HA antibody or the isotype-matched IgG. The precipitates were subjected to SDS-15%PAGE followed by WB using the Flag antibody or the HA antibody. 10% of nuclear extracts was used as input control.