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. 2021 Sep 2;11:17546. doi: 10.1038/s41598-021-97006-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Erk mediates DRP1 phosphorylation and LPS-induced mitochondrial fission. (A) HLMVECs were pretreated with PD98059 for 30 min followed by LPS for 30 min, phosphorylation of DRP1 was analyzed by Western blot. (B) Results of quantitative analysis are shown as intensity ratio of phosphorylated DRP1 to total DRP1. Values are means ± SD of 3 independent experiments. **p < 0.01, LPS versus LPS + PD98059. (C) HLMVECs were pretreated with PD98059 for 30 min followed by adding of MitoTracker 10 min before imaging. (D–G) Mitochondrial dynamics was assessed by number of individual fragments, number of networks, rod/branch length, and branch counts. Data were presented as means ± SD, for number of individual fragments, *p < 0.05, LPS alone versus LPS + PD98059; for number of networks, **p < 0.01, LPS alone versus LPS + PD98059; for rod/branch length, ***p < 0.005, LPS alone versus LPS + PD98059; for branch counts, *p < 0.05, LPS along versus LPS + PD98059. Presents are representative of at least 5 images. Scale bar 5 μm. (H) HLMVECs were treated same as described above and staining with MitoSOX for 10 min before imaging. Shown are representative of at least 5 images. Scale bar 10 μm. (I) MitoSOX intensity analysis, *p < 0.05, LPS alone versus LPS + PD98059.