FIG. 2.
Soraphen A sensitivity of the hpr1Δ mutant. (A) Strains with indicated genotypes were streaked onto YEPD medium (top) and YEPD medium containing 0.25 mg of soraphen A per ml (bottom) and incubated for 2 days at 30°C. wt, wild type. (B) Dose-response curves to soraphen A. The exponential growth rate was established after a 3-h lag period for various concentrations of soraphen A and is expressed as a percentage of the growth rate in the absence of soraphen A. The results for two strains of each genotype were averaged and plotted against the concentration of soraphen A. Curves are the best fit calculated with respect to Hill’s equation (Hill coefficients, 3 to 4). Calculated 50% infective doses are 0.09 mg/ml for the wild type and 0.04 mg/ml for both the acc1cs and the hpr1Δ strains.