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acc1ts is synthetically lethal with hpr1Δ. An acc1ts strain (YRXS12) was crossed with an hpr1Δ strain (U768-4C), diploids were sporulated, and tetrads were dissected. Four spores of a complete tetrad were replica plated on YEPD plates (top), YEPD supplemented with fatty acids (middle), and YEPD plates supplemented with 1 M sorbitol (bottom) and incubated at 30°C for 3 days. The relevant genotype of the spores is indicated on top. wt, wild type.
