FIG. 4.

Western blot analysis of biotinylated Acc1p levels in wild-type and hpr1Δ cells. Equal amounts of protein (10 μg) isolated from a whole-cell lysate were separated by SDS-gel electrophoresis, blotted, and probed with peroxidase-labeled avidin to detect biotinylated Acc1p and pyruvate carboxylase (Pyc) and anti-GAPDH or anti-Acc1p antibodies. Signal intensities were quantified and are listed in Table 2.