FIG. 6.
Northern analysis of CRY1 mRNA processing in wild-type, hpr1Δ, and acc1cs cells. Ten micrograms of total RNA isolated from cells grown at the permissive temperature (23°C) or shifted for 4 h to a nonpermissive temperature (37 or 17°C) was analyzed by Northern hybridization with CRY1 as a probe. The position of mature CRY1 mRNA (540 bases) is shown. The position of CRY1 pre-mRNA (847 bases) is indicated by the arrow. Oversized transcripts detected in hpr1Δ cells are indicated by the arrowheads pointing to the right. wt, wild type.