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A. Microscopic image of hMSCs; B. Scale of hMSCs culture; C. Experimental schedule. The primary cultured MSCs (2 x 106 cells/flask) were seeded in 75 cm2 culture flasks (total of 50 flasks). C. After culturing in complete media, culture medium was replaced with serum-free antibiotic-free medium for 48 h, and the culture supernatant was harvested to isolate microvesicles.
