Figure 4. Enrichment of microvesicles by sucrose gradient ultracentrifugation.

A. A discontinuous sucrose density gradient was prepared by layering 1.5 ml of 0.8 M sucrose gradient upon 0.5 ml of 2.7 M sucrose density solution. Next, 33 ml of concentrated conditioned medium was added to the top lay carefully. B. After centrifugation, microvesicles are enriched in the interface between medium and sucrose layers.