Figure 3. 1 x 10⁵ human neutrophils (hNeu) were cultured alone or with adipose tissue derived mesenchymal stem cells (MSC) at hNeu:MSC ratios of 1,000:1, 100:1 and 10:1 and subsequently activated with 100 ng/ml PMA for 3 h.

Cocultures were labelled with 0.25 µM Sytox orange for 5 min at room temperature and fluorescence intensities were measured by a microplate reader. Culture medium containing Sytox orange served as background fluorescent control. Fluorescence intensities were normalized on the intensity of non-activated controls. Results are expressed as mean ± SEM of quintuplicate measurements. This experiment was independently repeated three times with similar results. **P < 0.01; ***P < 0.001; RFI, relative fluorescence intensity; AU, arbitrary unit.