Components	Final concentration	Stock concentration	Volume of stock solution/weight for 1 L
KH2PO4	2 mM	40 mM	50 ml
NaNO3	5 mM	100 mM	50 ml
MgSO4	2 mM	40 mM	50 ml
CaCl2	1 mM	500 mM	2 ml
Fe-EDTA	50 μM	10 mM	5 ml
H3BO3	50 μM	50 mM	1 ml
MnSO4	12 μM	12 mM	1 ml
ZnCl2	1 μM	1 mM	1 ml
CuSO4	1 μ M	1 mM	1 ml
Na2MoO4	0.2 μM	0.2 mM	1 ml
Sucrose	1%		10 g
MES	0.5 g/L		10 g
Agar	0.8%		8 g
pH 5.7 (adjust with 1 N NaOH)