Figure 1. Number of intracellular bacteria in Dictyostelium cells co-incubated with a wild type strain of E. coli (W3110) and with the mutant, E345, with triple gene deletion.

The assay was performed at MOI 1. Data were analyzed with a t-test and the two strains were significantly different at the 1% level (P = 0.0045, n = 6 for each strain). Further details and examples can be found in Hao et al., 2016 .