Figure 3. 96-well plate set-up for pseudotype titration.

Serial dilution step showing addition of 100 µl of pseudotype virus supernatant to each well of row A and dilution of 50 µl taken from this well to row B. This process is continued to end of plate (row H) at which point the final 50 µl is discarded. Delta Env control is indicated in red (column 11) and cell only control is indicated in green (column 12). One set of pipette tips can be used per dilution series (plate).