Table 1. Analysis of PV titration data.
RLU values are multiplied to give an RLU/ml value (example highlighted in green) giving RLU/ml values for each of the dilution points. The mean/average is then calculated from all 8 dilution points. Care must be taken to observe a linear relationship between dilution factor (X factor) and RLU, or multiplication can lead to inflated RLU/ml values (example highlighted in yellow).
| X factor | SARS-S | VSV-G | Virus X |
|---|---|---|---|
| 20 | 5.58E+05 | 7.17E+08 | 3.05E+03 |
| 40 | 2.90E+05 | 5.63E+08 | 1.10E+03 |
| 80 | 1.43E+05 | 3.79E+08 | 6.29E+03 |
| 160 | 6.17E+04 | 2.16E+08 | 8.80E+02 |
| 320 | 3.10E+04 | 1.36E+08 | 5.43E+03 |
| 640 | 2.41E+04 | 6.18E+07 | 3.39E+03 |
| 1280 | 7.15E+03 | 3.06E+07 | 1.96E+03 |
| 2560 | 3.62E+03 | 1.44E+07 | 3.33E+03 |
| RLU/ml | 1.12E+07 | 1.43E+10 | 6.11E+04 |
| 1.16E+07 | 2.25E+10 | 4.40E+04 | |
| 1.14E+07 | 3.03E+10 | 5.03E+05 | |
| 9.87E+06 | 3.46E+10 | 1.41E+05 | |
| 9.91E+06 | 4.35E+10 | 1.74E+06 | |
| 1.54E+07 | 3.96E+10 | 2.17E+06 | |
| 9.16E+06 | 3.92E+10 | 2.51E+06 | |
| 9.26E+06 | 3.69E+10 | 8.54E+06 | |
|
Average RLU/ml |
1.10E+07 | 3.26E+10 | 1.96E+06 |