Figure 1. Tool for apoplastic wash collection.

Apoplastic wash is collected by packaging whole Arabidopsis thaliana rosettes in a 30 ml syringe. Eight additional holes are melted into the end of the syringe with forceps to create channels for the wash to flow through. A 2.5 cm hole is punched out of a 50 ml conical tube cap. The cap is then slid onto the syringe 2 cm from the top. This allows the syringe to be screwed securely into a 50 ml conical tube. See Video 1.