Table 1. Example of raw data from DiOC6 fluorescence quantification.
The following data was generated from a replicate of an experiment presented in Rutter and Innes (2017). The experiment compared EV secretion between mock-treated Arabidopsis thaliana plants (Positive Control) and plants infected with a virulent strain of Pseudomonas syringae (Test).
| Sample | replicate | reading 1 | reading 2 | reading 3 | avg. | avg. blank | –avg. blank |
(avg. pos.
–avg. blank)
|
/(avg. pos.
–avg. blank)
|
avg. X/(avg. pos. –avg. blank) |
|---|---|---|---|---|---|---|---|---|---|---|
| Blank | 1 | 457.5 | 439.5 | 433 | 443.3333333 | 444.5444444 | ||||
| 2 | 456.9 | 451.2 | 442.6 | 450.2333333 | ||||||
| 3 | 457.1 | 431.6 | 431.5 | 440.0666667 | ||||||
| Positive Control | 1 | 2096.7 | 1653.1 | 2523.2 | 2091 | 1646.455556 | 1515.655556 | 1.086299291 | 1 | |
| 2 | 2085 | 1516 | 2323.3 | 1974.766667 | 1530.222222 | 1.009610803 | ||||
| 3 | 1556.7 | 1570.7 | 2317.1 | 1814.833333 | 1370.288889 | 0.904089906 | ||||
| Test | 1 | 4073.9 | 5160.6 | 3786.1 | 4340.2 | 3895.655556 | 2.570277621 | 2.584294291 | ||
| 2 | 4385.9 | 5328.7 | 3916.9 | 4543.833333 | 4099.288889 | 2.704630926 | ||||
| 3 | 4030.8 | 4762.8 | 3807.3 | 4200.3 | 3755.755556 | 2.477974327 |