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. 2021 Aug 20;11(16):e4121. doi: 10.21769/BioProtoc.4121

Figure 5. Monitoring protein splicing of Mycobacterium smegmatis DnaBi1 intein using in-gel fluorescence with Tris-Glycine SDS-PAGE.

Figure 5.

Presence of 100 μM zinc acetate inhibits splicing. Left, Coomasssie stained. Right, sfGFP signal. sfGFP-tagged products are indicated as Precursor (P), Ligated exteins (LE), and Intein-C-extein (IC).