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. 2017 Aug 5;7(15):e2425. doi: 10.21769/BioProtoc.2425

Figure 3. Dumbbell vectors for enhanced gene expression.

Figure 3.

Left side. Design of advanced dumbbell vectors: db-luc: basic vector; db-luc-enh: dumbbell featured with the SV40 enhancer; db-int-luc: dumbbell featured with an intron; db-int-luc-enh: dumbbell featured with SV40 enhancer and intron. Right side. Analytical 0.8% agarose gel electrophoresis of the four luciferase dbs after exonuclease treatment. The expected dumbbell bands but no by-products are detected. Numbers of lanes on the right correspond to the dumbbell numbers on the left. M: GeneRuler DNA Ladder Mix (Thermo Fisher Scientific).