Figure 1.

NAT10 expression and the ac4C content of total RNA are decreased in osteoporosis bone tissues

(A and B) mRNA expression of NAT10 in femurs from osteoporosis patients and OVX mice was decreased compared with expression in their corresponding controls, as determined by quantitative real-time PCR, n = 6. (C–F) Decreases in the ac4C content in total RNAs extracted from femurs of osteoporosis patients and OVX mice compared with in the corresponding control determined by ac4C dot blot analysis. (D) Densitometry quantitation of (C). (F) Densitometry quantitation of (E), n = 6. (G–J) The protein level of NAT10 in femurs from osteoporosis patients and OVX mice was decreased compared with expression in their corresponding controls, as determined by western blotting. (H) Densitometry quantitation of (G). (J) Densitometry quantitation of (I), n = 6. (K and L) The expression of NAT10 in the femurs of osteoporosis patients and OVX mice was decreased relative to that in their control groups, as determined by immunofluorescence staining (scale bars: 200 μm), n = 6. All data are expressed as the means ± SD. ∗p < 0.05, ∗∗p < 0.01 (n = 3 independent experiments).