Figure 3.

Remodelin, an inhibitor of NAT10, aggravates bone loss in OVX mice

(A) Illustration of Remodelin treatment or solution alone for OVX mice or sham mice, n = 6. (B and C) The protein level of NAT10 was decreased in Remodelin treatments group compared with the solution alone group, as determined by western blotting, (C) Quantification of band intensities (B), n = 6. (D) The ac4C level of total RNA was decreased in the Remodelin treatments group compared with the solution alone group, as determined by western blotting, n = 6. (E) Densitometry quantitation of (D), n = 6. (F) Representative μCT images of four groups of mice, n = 6. (G–K) Quantification of bone microstructural parameters of BV/TV (G), Tb.N (H), Tb.Th (I), Tb.Sp (J), and BSA/BV (K), n = 6. (L and M) OVX mice treated with Remodelin demonstrated aggravated bone loss, but the bone mass of sham mice treated with Remodelin had no difference from sham mice treated with solution alone (scale bars: 100 μm), as determined by H&E staining, n = 6. (N) Quantification of the trabecular area from four groups of mice, n = 6. All data are expressed as the means ± SD. ∗p < 0.05, ∗∗p < 0.01 (n = 3 independent experiments).