Figure 4.

The ac4C content of total RNA and NAT10 expression are elevated when BMSCs are cultured in OM rather than PM

(A and B) BMSCs cultured in OM formed more calcium nodules than those cultured in PM from days 7 to 14, as determined by ARS staining (scale bar: 250 μm), n = 6. (C and D) BMSCs cultured in OM synthesized more ALP than BMSCs cultured in PM from days 7 to 14. During osteoblast differentiation, ALP staining peaked on day 10, as determined by ALP staining (scale bar: 250 μm), n = 6. (E) The protein level of NAT10 was increased in BMSCs cultured in OM compared with PM, n = 6. (F) Quantification of band intensities, n = 6. (G) The ac4C level of BMSCs cultured in OM increased compared with PM, as determined by dot blot analysis, n = 6. (H) Densitometry quantitation of (G), n = 6. All data are presented as the means ± SD. ∗p < 0.05, ∗∗p < 0.01 (n = 3 independent experiments).