Figure 6.

Identification of NAT10 targets through acRIP-seq

(A) Schematic illustration of acRIP-seq performed in two healthy donor BMSCs, which were divided into two groups shown in the picture (two biological repeats per group). (B) Enriched motifs of ac4C peaks within two groups of BMSCs. (C) Pie charts show the percentage of ac4C peak distribution in the acetylated transcripts. (D) Volcano map of differentially expressed ac4C gene peaks in BMSCs transfected with siNAT10 versus siNC. (E) GO enrichment analysis of the differentially expressed ac4C gene peaks. (F) The acRIP-seq assay showed the ac4C peaks on RUNX2 mRNA between the two groups. ac4C sites (red) were indicated in the 3′ UTR. p = 2.04E-09.