Figure 1.

The expression of lncRNA XIST is upregulated and correlates with NIHSS scores after CIS

(A) Serum levels of lncRNA XIST in acute CIS patients and healthy controls were measured using qRT-PCR. Results are expressed as the mean ± standard deviation. Note that the serum levels of lncRNA XIST in CIS patients (n = 77) decreased at day 2 but increased at day 7 after ischemic onset as compared to healthy controls (n = 60). (B) The Pearson analyses revealed that serum levels of lncRNA XIST were significantly negatively correlated with NIHSS scores at day 7 after ischemic onset in acute CIS patients (n = 77). (C) The expression of lncRNA XIST in the ipsilateral ischemic cerebral cortex from sham-operated mice (sham; S, control) or mice at days 0, 1, 2, 4, and 7 post-ischemia was examined by qRT-PCR. Results are expressed as the mean ± standard deviation (n = 6 per experimental group). Note that the expression of lncRNA XIST decreased during the first 2 days post-ischemia but then increased between 4 and 7 days post-ischemia. (D) qRT-PCR analysis for lncRNA XIST levels after BECs was subject to OGD/R. Results are expressed as the mean ± standard deviation (n = 4 per experimental group). Note that within the first 6 h of OGD, lncRNA XIST levels decreased but then by 24–72 h, lncRNA XIST levels markedly increased compared to pre-OGD control levels. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with control.