Figure 6.

LINC00941 interacted with mammalian STE20-like protein kinase 1 (MST1) to trigger the Hippo pathway

(A) ISH fluorescence staining of LINC00941. (B) RNA pull-down assay with LINC00941, followed by Coomassie staining. (C) RNA pull-down assay with LINC00941 and LINC01234, followed by MST1 immunoblots. (D and E) RNA-binding protein immunoprecipitation (RIP) assay for MST1, followed by LINC00941 agarose gel electrophoresis and qPCR (E). (F) RNA pull-down assay for full-length or truncated LINC00941, followed by immunoblots using the MST1 antibody. (G) Competition assays using biotin-labeled and unlabeled LINC00941. (H) Immunofluorescence staining of MST1 and LINC00941. (I) CoIP assays were performed with MST1 antibody in LINC00941 control and knockdown cells, followed by PP2A, striatin 3 (STRN3), and MST1 detection by WB. (J) ECAR analysis of control or PP2A knockdown PANC-1. (K) Cell viability assay of control or PP2A knockdown PANC-1. All statistical significances were calculated using two-tailed Student’s t tests to compare with the NC or control group. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.