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. 2021 Jun 22;11(8):e2266. doi: 10.1002/brb3.2266

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© 2021 The Authors. Brain and Behavior published by Wiley Periodicals LLC

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fasudil reversed KA‐induced inhibition of neurite outgrowth and spines in Neuro‐2a cells. (A, B) Representative intrinsic optical and rhodamine‐phalloidin immunofluorescent images of Neuro‐2a cells, respectively. Scale bar = 20 μm. (C, D) Quantitative analysis of the length of outgrowth in Neuro‐2a cells under the observation of intrinsic optical microscope and rhodamine‐phalloidin immunofluorescence, respectively. (E) Quantitative analysis of the number of spines in Neuro‐2a cells. *p < .05 by one‐way ANOVA compared to control group (n = 100). #p < .05 by one‐way ANOVA compared to KA‐treated group (n = 100)