TABLE 1.

Pairs of oligonucleotide primers used in the nTRAP assay

Primer	Sequencea		Size (nt)
	Upstream	Downstream
Modified TSb
nTS-CTT	5′-AATCCGTCGAGCAGACTT-3′	-------Telomerase extension------→	18
nCX	←-----PCR amplification-----	3′-AATCCGTATCCGTATCCGTATCCG-5′	24
Telomere addition site
me8-TTA	5′-TTTGGTTATTTTATCTAAATTTTA-3′		24
TCP-23		3′-GAATCCGAATCCTTAAGCTATAG-5′	23
Terminal telomeric permutations of nTS
nTS-GCT	5′-AATCCGTCGAGCAGAGCT-3′		18
nTS-CTT	5′-AATCCGTCGAGCAGACTT-3′		18
nTS-TTA	5′-AATCCGTCGAGCAGATTA-3′		18
nTS-TAG	5′-AATCCGTCGAGCAGATAG-3′		18
TCP-CC		3′-CCGAATGCTCTGCTTAAGCTATAG-5′	24

^aUpstream primers (i.e., telomerase substrate) are shown in the 5′-to-3′ orientation, and their 3′-terminal telomeric nucleotides are boldfaced. Downstream primers are shown in the 3′-to-5′ orientation, and those of their nucleotides that are complementary to the sequence synthesized by telomerase are boldfaced. The 3′-terminal bases of the upstream primers that may induce primer dimer formation with the downstream primers are underlined (see the text). 

^bTS, telomerase substrate.