Figure 1.

Differential expression and localization of 5′-tiRNA-Cys-GCA in vivo and in vitro

(A) The location of 5′-tiRNA-Cys-GCA in VSMCs was detected by FISH in human aortic and AD samples. (B) Quantification of the relative expression of 5′-tiRNA-Cys-GCA in (A). (C) Quantitative real-time PCR analysis of 5′-tiRNA-Cys-GCA expression in human aortic dissection (AD) and normal aortic tissues. (D) Quantitative real-time PCR was used to analyze 5′-tiRNA-Cys-GCA expression of VSMCs treated with ox-LDL over time gradient. Data are presented as mean ± SD. Each experiment was repeated at least three times. Scale bars, 200 μm. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.