Figure 2.

Highly expressed MIF promotes TMZ resistance in glioma cells

(A and B) qRT-PCR and western blot showed that the expression of MIF in U87TR/251TR cells was significantly upregulated compared with U87/251 cells. (C and D) CCK-8 showed that the IC₅₀ values were significantly deceased after MIF knockdown in TR cells. In contrast, overexpressed MIF significantly increased the IC₅₀ values in TS cells. (E and F) The flow cytometry (FCM) showed that loss of MIF in TR cells significantly increased cell apoptosis upon TMZ treatment for 48 h (50 μg/mL). (G and H) On the contrary, TUNEL assay showed that overexpression of MIF may cause apoptosis rates to decrease in TS cells. (I–L) The percentage of the EdU-positive cells were significantly decreased in TR-MIF-sh groups than in TR-NC groups. On the other hand, the percentage of the EdU-positive cells were significantly increased in TS-MIF-OE groups than in TS-NC groups. ^∗∗p＜0.01, ^∗∗∗p＜0.001, ^∗∗∗∗p＜0.0001.