Figure 4.

Exosomal MIF confers TMZ resistance via promotion of proliferation and inhibition of apoptosis in recipient cells

(A and B) The FCM results showed that the apoptosis rates in TR EXO and TR-NC EXO groups were significantly lower than in PBS and TR-MIF-sh EXO groups (TMZ = 50 μg/mL). (C) EdU assay demonstrated that TR EXO and TR-NC EXO groups had a higher cell proliferation rate than PBS and TR-MIF-sh EXO groups (scale bars, 50 μm). (D) EdU statistics. (E) CCK-8 showed that TR EXO and TR-NC EXO significantly enhanced the TMZ resistant characteristic in TS cells. (F) Western blot for caspase-3 and caspase-9 expression in the different treated glioma cells. ^∗∗p＜0.01, ^∗∗∗p＜0.001, ^∗∗∗∗p＜0.0001.