Figure 3.

Tumor-infiltrating lymphocytes in subcutaneous AKR tumors treated with the combination of G47Δ and CTLA-4 inhibition

C57BL/6 mice harboring subcutaneous AKR tumors were treated according to the schedule shown in Figure 2A. Tumor-infiltrating immune cells were analyzed by flow cytometry 7 days after the initial treatments. Comparisons of absolute numbers of (A) CD3 cells, (B) CD4⁺ T cells, (C) CD8⁺ T cells, and (D) Tregs (CD4⁺Foxp3⁺) per gram of tumor tissue. (E) The percentage of Tregs, gated on CD4⁺ T cells. (F) The ratio of CD8⁺ T cells to Tregs. (G) The percentage of CD3, CD8⁺, CD4⁺, and Tregs gated on CD45 cells. Results are representative of two independent experiments with 7 animals per group, and bars represent the SEM. One-way ANOVA followed by Dunnett’s test was used to determine statistical significance (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant).