Figure 3.

Determination of the on-target DNA editing efficiency of CABE-RY in HEK293T cells

(A) Relative C-to-G and A-to-T base editing efficiencies of CABE-RY with different PAMs at 16 human genomic target DNA sites (n = 3 independent replicates). The error bars represent the SEM values. (B) Sequencing analysis of the base editing window of CABE-RY across NNN PAMs in HEK293T cells. The error bars represent the SEM values. The data are presented for each edited base separately at 48 endogenous sites (n = 3 independent replicates). (C) Aggregate distribution of C-to-T (red) and A-to-G (blue) edits made across the editing window with CABE-RY. The error bars represent the SEM values. The data are presented for each edited base in the editing window at 48 endogenous sites.