FIG. 3.

Mapping of acetylation sites by using synthetic GATA-1 peptides. Peptides corresponding to residues 236 to 255 containing the N-motif (top) and residues 304 to 323 containing the C-motif (bottom) of murine GATA-1 were acetylated by CBP-HAT in vitro and sequenced. Following purification and Edman degradation, [¹⁴C]acetate incorporation into individual amino acids was determined by scintillation counting. The RXKK and KXKK motifs are boxed. Note that the absolute amounts of [¹⁴C]acetate incorporation is significantly higher in the C-terminal peptide.