FIG. 5.

(A) Acetylation of GATA-1 does not affect DNA binding. Gel mobility shift assays of acetylated and nonacetylated GST–f(GATA-1). Acetylation reactions were performed in the presence or absence of acetyl-CoA. The indicated amounts of protein were used in a gel mobility shift assay with a probe containing a single GATA site. (B) (Top) The lysine-rich motifs of GATA-1 do not participate in DNA binding. Gel mobility shift assays with wild-type (wt) and mutant GATA-1 proteins expressed in COS cells. NA and CA represent GATA-1 mutants bearing alanine substitutions in the N- and C-motifs, respectively; NR and CR represent analogous mutants with arginine substitutions; MEL cell nuclear extracts served as positive control. (Bottom) A control Western blot shows similar levels of GATA-1 expression.