Figures 18 and 19.

NSG-SGM3 mice implanted with transduced hCD34-positive cells. Figure 18. Leukemia, bone marrow, mouse no. 15. (a,b) A cross-section of a vertebra, with replacement of the marrow by leukemic cells, which infiltrate into the epidural space and surrounding muscle. HE. Inset: the leukemic cells are hNuMA-positive. The leukemia is myelomonocytic, as indicated by positivity for MPO (c) and hCD68 (d). Figure 19. Leukemia as well as macrophage and lymphocyte infiltrates, liver, . (a) There are aggregates of large macrophages with cytoplasmic pigment, and admixed small lymphocytic cells. HE. (b) Intracytoplasmic erythrocytes indicative of erythrophagocytosis. (c) The lymphocytes and macrophages are immunolabeled for hNuMA1. (d) Multiple aggregates of macrophages containing blue-staining iron. Inset: higher magnification. Prussian blue. (e–h) These cells are positive for hCD68 (e) and hCD163 (f) and are rimmed by mouse F4/80-positive cells (g), similar to the cells of the humanized mice in Figure 14. Occasional GFP-positive leukemia cells are present in the liver sinusoids (h, purple chromogen, arrows). (i, j) Admixed with the macrophages are low numbers of CD3-positive T cells (i) and PAX5-positive B cells (j).