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. 2021 Aug 15;11(8):3857–3876.

Figure 1.

Figure 1

GSN 3’UTR could suppress the promotional effect of GSN protein on the growth, migration and invasion of NSCLC cells in vitro. H1299 (A) and A549 (B) cells were transfected with Flag-vector, Flag-GSN and Flag-GSN-3’UTR plasmids, and analyzed by western blotting. Signal intensities were quantified with GAPDH as internal control. (C) The effects of Flag-vector, Flag-GSN and Flag-GSN-3’UTR overexpression on the proliferation of H1299 (upper panel) and A549 cells (lower panel), analyzed by MTT assay. (D, E) H1299 and A549 cells were transfected with the indicated plasmids, and the colony formation ability was quantified by a colony formation assay. (F, G) Transwell migration assays of H1299 and A549 cells transfected with the indicated plasmids. (H, I) Transwell invasion assays of H1299 and A549 cells transfected with the indicated plasmids. Data are presented as the mean ± s.d. and are representative of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.