Figure 3.

GSN 3’UTR localized the exogenously co-expressed GSN to the membrane. Subcellular location of Flag-GSN protein by immunofluorescence confocal microscopy. H1299 (A) and A549 (B) cells transfected with the indicated plasmids were stained with appropriate primary and secondary antibodies. DAPI was used for nuclear staining. F-actin was stained using rhodamine-conjugated phalloidin. Scale bars: 10 μm. (C, D) The counting of cells displaying various Flag-GSN location shown in (A and B). Data shown were from three independent experiments (> 15 cells each, n=3).