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. 2021 Aug 15;11(8):3857–3876.

Figure 4.

Figure 4

The functions of GSN 3’UTR could be independent of the exogenously co-expressed GSN protein. A, B. Cell migration ability of H1299 and A549 cells transfected with Luc-vector or Luc-3’UTR was analyzed by transwell assay. C, D. Invasion ability of H1299 and A549 cells transfected with Luc-vector or Luc-3’UTR was measured by use of transwell assay. E. Transfected of GSN 3’UTR in H1299 and A549 cells decreased the proliferation rate by MTT assay. F. The level of E-cadherin, Vimentin and GAPDH was examined by western blotting analysis in H1299 and A549 cells transfected with Luc-vector or Luc-3’UTR. G. The level of Vimentin/GAPDH and N-cadherin/GAPDH was examined by RT-qPCR. Data are presented as the mean ± s.d. and are representative of at least three independent EXPERIMENTS. *P < 0.05, **P < 0.01, ***P < 0.001.