Figure 7.

Exogenous GSN 3’UTR inhibits the EMT process and migration/invasion abilities of NSCLC cell through interaction with Tra2β and perturbation of endogenous GSN protein localization. A. Without GSN 3’UTR transfection, endogenous GSN proteins are distributed in the cytoplasm, Tra2β protein is exclusively located in the nucleus, with actin-rich stress fibers across the cell body, and cells display mesenchymal characteristics with high mobility. B. Upon GSN 3’UTR transfection, through an unknown mechanism, Tra2β protein is translocated from the nucleus to the cytoplasm, where it can interact with the endogenous or exogenous GSN 3’UTR, bringing the corresponding mRNA to the proximity of the plasma membrane and eliciting the localized translation of the latter. This in turn perturbs the remodeling of actin-rich cytoskeletons, causing disappearance of stress fibers, and inhibits the EMT process, thereby decreases the mobility of the cell.