Figure 3.

Combination treatment induces cell apoptosis in trastuzumab-resistant HER2-positive cell line. A. Jimt1 cells were treated with DMSO, TRZ (5 µg/ml), TTF, combination for 48 h. Immunoblotting was performed on cell lysates (30 µg) with antibodies against pHER2, HER2, Cleaved PARP, Bcl-2, and β-actin. B. Representative of TUNEL. Data are presented as per the mean ± standard error of the mean (n = 10), *P < 0.05. C. TUNEL-positive cells were stained and imaged with 10 × objective lenses using in cell analyzer. Apoptotic cells are shown in red.