Figure 1.

PANC-1/GR and SW1990/GR cells displayed an EMT phenotype. A, B. MTT assay was used to determine the cytotoxicity of gemcitabine to PANC-1, PANC-1/GR, SW1990, and SW1990/GR cells. IC₅₀ values of gemcitabine in each cell line were calculated. C, D. Transwell assay was performed to determine the cell migration and invasion capacities of GR cells (PANC-1/GR and SW1990/GR) and their respective parental cells. E, F. The mRNA and protein levels of EMT molecular markers including E-cadherin, N-cadherin, Vimentin, Snail, ZEB1, and ZEB2 were detected using qRT-PCR and western blot analysis, respectively. At least three independent experiments were performed in each group and mean ± SD was used to represent the final result. *P<0.05, **P<0.01, ***P<0.001.