Figure 2.

Downregulation of NEAT1 enhanced the cytotoxicity of gemcitabine to SW1990/GR and PANC-1/GR cells. A. Relative expression of NEAT1 in GR cells (PANC-1/GR and SW1990/GR) and their respective parental cells. B. Relative expression of NEAT1 in GR cells after transfection with NEAT1 shRNA or negative control shRNA. C, D. MTT assay was used to determine the cytotoxicity of gemcitabine to GR cells after transfection with NEAT1 shRNA or negative control shRNA. IC₅₀ values of gemcitabine in each group were calculated. E, F. The cell apoptosis in GR cells (PANC-1/GR and SW1990/GR) after NEAT1 knockdown or/and gemcitabine treatment was detected using flow cytometry analysis (gemcitabine: 5 μM, 48 h). At least three independent experiments were performed in each group and mean ± SD was used to represent the final result. *P<0.05, **P<0.01, ***P<0.001.