Downregulation of NEAT1 reversed the EMT process in SW1990/GR and PANC-1/GR cells. A, B. Transwell assay was performed to determine the cell migration and invasion capacities of the GR cells after transfection with NEAT1 shRNA or negative control shRNA. C, D. The mRNA and protein levels of EMT molecular markers including E-cadherin, N-cadherin, Vimentin, Snail, ZEB1, and ZEB2 in GR cells after transfection with NEAT1 shRNA or negative control shRNA were detected using qRT-PCR and western blot analysis, respectively. At least three independent experiments were performed in each group and mean ± SD was used to represent the final result. *P<0.05, **P<0.01.