Skip to main content
. 2021 Aug 15;11(8):3841–3856.

Figure 5.

Figure 5

NEAT1 regulated ZEB2 expression by sponging miR-506-3p in SW1990/GR and PANC-1/GR cells. A. Relative expression of miR-506-3p and ZEB2 in GR cells (PANC-1/GR and SW1990/GR) and their respective parental cells. B. Relative expression of miR-506-3p and ZEB2 in GR cells after transfection with NEAT1 shRNA or negative control shRNA. C, D. The predicted binding sites of miR-506-3p in the 3’-UTR of NEAT1 or ZEB2 were determined using Starbase v3.0 or TargetScan. Luciferase reporter assays showed that miR-506-3p overexpression significantly suppressed the activity of the reporter containing wild-type NEAT1 or wild-type ZEB2 in GR cells (PANC-1/GR and SW1990/GR). E. The mRNA and protein levels of ZEB2 in GR cells after miR-506-3p overexpression were detected using qRT-PCR and western blot analysis, respectively. F. qRT-PCR and western blot analysis of the expression of ZEB2 in GR cells transfected with NEAT1 shRNA in the presence of miR-506-3p inhibitor or NC. At least three independent experiments were performed in each group and mean ± SD was used to represent the final result. *P<0.05, **P<0.01, ***P<0.001.