Figure 6.

The function of NEAT1 in SW1990/GR and PANC-1/GR cells was dependent on miR-506-3p. A, B. The MTT assay was used to determine the cytotoxicity of gemcitabine to GR cells after transfection with miR-506-3p mimic or NC. IC₅₀ values of gemcitabine in each group were calculated. C, D. Flow cytometry analysis of gemcitabine induced cell apoptosis in GR cells after transfection with miR-506-3p mimic or NC. Cells in each group were exposed to 5 μM gemcitabine for 48 h. E, F. MTT assay was used to determine the cytotoxicity of gemcitabine to GR cells transfected with NEAT1 shRNA in the presence of miR-506-3p inhibitor or NC. IC₅₀ values of gemcitabine in each group were calculated. G, H. Flow cytometry analysis of the gemcitabine-induced cell apoptosis in GR cells transfected with NEAT1 shRNA in the presence of miR-506-3p inhibitor or NC. Cells in each group were exposed to 5 μM gemcitabine for 48 h. At least three independent experiments were performed in each group and mean ± SD was used to represent the final result. *P<0.05, **P<0.01, ***P<0.001.