Downregulation of NEAT1 improved the chemotherapeutic effect of gemcitabine in nude mouse xenograft models. A. Representative images of xenograft tumors in each group, including sh-NC + vehicle, sh-NEAT1-1 + vehicle, sh-NC + Gemcitabine and sh-NEAT1-1 + Gemcitabine. B, C. Tumor growth curves and tumor weights of each group. D. qRT-PCR analysis of the expression of NEAT1 in the two tumor xenografts groups. E, F. qRT-PCR and western blot analysis of the expression of E-cadherin, N-cadherin, Vimentin, Snail, ZEB1, and ZEB2 in the two tumor xenografts groups. G. TUNEL staining images for apoptosis in the two tumor xenografts groups. At least three independent experiments were performed in each group and mean ± SD was used to represent the final result. *P<0.05, **P<0.01, ***P<0.001.