Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 13;12:708034. doi: 10.3389/fphar.2021.708034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Liu, Liang, Zhu, Zhou, Wang, Wang, Wu, Goh and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Target genes of miR-3068-5p in NAc of mice involving in METH sensitization. (A) A total of 208 overlapping genes between the METH-induced genes in our previous study (https://www.ebi.ac.uk/arrayexpress/, E-MTAB-2843) and the predicted target genes of miR-3068-5p were identified and subjected to IPA analysis. (B) Identification of the main neurological dysfunctions of the 208 genes identified from (A). (C) IPA analysis identified five overlapping genes from (A) relevant to hyperactive behavior, release of catecholamine, release of dopamine, development of neurons, quantity of dendritic spines, synaptic transmission, release of neurotransmitters, development of the body axis, long-term synaptic depression of neurons, neurotransmission, anxiety, long-term potentiation, long-term synaptic depression of synapses, and synaptic depression. (D) mRNA expression of the five predicted target genes of miR-3068-5p relevant to locomotion following miR-3068-5p sponging in the NAc of METH-sensitized mice. (E, F) Graphs show the expression of Grin1 mRNA upon overexpression (E) or knockdown (F) of Ago2. Student’s t-test: *p < 0.05 and **p < 0.01 compared to the AAV control group. The data are presented as the mean ± SEM, n = 6–8. (G) Graphs showing the dual-luciferase activities upon transfection of Grin1 Wt or mutant (Mu) expression conducted alone (NC) or by cotransfection with mimic-miR-3068-5p. Two-way ANOVA: ***p < 0.001 compared to the corresponding NC group. The data are presented as the mean ± SEM, n = 6. NAc, nucleus accumbens; METH sensitization, METH-induced locomotor sensitization; IPA, Ingenuity Pathway Analysis.